Regulation of Cytoplasmic and Vacuolar Volumes by Plant Cells in Suspension Culture 1

AUTOR(ES)

Owens, Trevor

RESUMO

Quantitative microscopical measurements have been made of the proportion of cell volume occupied by cytoplasm in a cell suspension culture derived from cotyledons of bush bean (cv. Contender). On a 7-day culture cycle, the content of cytoplasm varies from 25% at the time of transfer to 45% at the start of the phase of rapid cell division. If the culture is continued beyond 7 days, the vacuole volume reaches 90% of cell volume by day 12.

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