Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion.
AUTOR(ES)
Sallus, L
RESUMO
The regulation of the fadL gene, which encodes a long-chain fatty acid (LCFA) transport component, was examined by constructing a strain of Escherichia coli K-12 that bears a phi (fadL-lacZ+) operon fusion plus a wild-type fadL gene. This merodiploid strain expressed LCFA transport and beta-galactosidase activity coordinately under noninducing, inducing, and catabolite-repressing conditions. Merodiploid strains which carried a defective fadR gene expressed LCFA transport and beta-galactosidase activity constitutively. These results suggest that expression of the fadL gene is regulated by the fadR gene and is inducible at the level of transcription by LCFA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217849Documentos Relacionados
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