Regulation of Herpesvirus Macromolecular Synthesis: Nuclear Retention of Nontranslated Viral RNA Sequences
AUTOR(ES)
Kozak, M.
RESUMO
We report two instances of selective accumulation of herpes simplex 1 RNA transcripts in different compartments of infected HEp-2 cells. In the first, transcripts derived from about 50% of the viral DNA accumulated in the nuclei of cells 8 hr after infection. However, only 40-42% of the DNA was represented in transcripts accumulating in both cytoplasm and polyribosomes. A more striking disparity in the distribution of transcripts between nuclei and cytoplasm occurred when viral infection was initiated and maintained for several hours in the absence of protein synthesis. RNA complementary to about 50% of the viral DNA accumulated in the nuclei, while transcripts derived from only about 10% of the DNA were detectable in the cytoplasm. The transcripts that were selectively transported in the presence of cycloheximide seem to be functional messenger RNA molecules, since they were found on polysomes immediately after cycloheximide reversal. In contrast, RNA retained in the nuclei during the period of cycloheximide treatment was not mobilized when protein synthesis subsequently resumed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433874Documentos Relacionados
- Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides.
- Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.
- Regulation of herpesvirus macromolecular synthesis. VI. Synthesis and modification of viral polypeptides in enucleated cells.
- Regulation of Herpesvirus Macromolecular Synthesis I. Cascade Regulation of the Synthesis of Three Groups of Viral Proteins 1
- Regulation of herpesvirus macromolecular synthesis: temporal order of transcription of alpha genes is not dependent on the stringency of inhibition of protein synthesis.