Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.
AUTOR(ES)
MacNeil, D
RESUMO
Strains with lac fused to each of the seven nif operons were isolated by two different methods. Repressing conditions prevented expression of all nif operons, whereas derepressing conditions led to the expression of all nif operons. No differences in Nif regulation were observed between Escherichia coli and Klebsiella pneumoniae with the same nif-lac fusions. Most derivatives of nif-lac fusion strains selected on lactose and NH4+ contained nif operator mutations. Some derivative contained deletions, which establishes that the direction of transcription of all seven nif operons is toward his
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217279Documentos Relacionados
- Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.
- Comparative organization of nitrogen fixation-specific genes from Azotobacter vinelandii and Klebsiella pneumoniae: DNA sequence of the nifUSV genes.
- Physical characterization of ilv-lac fusions.
- Physical map of chromosomal nitrogen fixation (nif) genes of Klebsiella pneumoniae
- Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.