Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein.

AUTOR(ES)
RESUMO

A chromosomal deletion beginning at a Tn10 located ca. 8 kilobases upstream from the ompC structural gene and extending through the 2.6-kilobase HindIII fragment carrying the ompC was isolated. The 2.6-kilobase ompC fragment was cloned into lambda 540 to obtain phage lambda 540C1. When the deletion mutant was lysogenized with lambda 540C1, the resulting strain produced normal levels of OmpC protein, and expression of this protein was regulated by osmolarity, carbon source, and the lc gene of phage PA-2, indicating that the cloned fragment contained all of the information required for regulated expression of ompC. The strain carrying the deletion was partially constitutive for expression of OmpF protein, whereas the lambda 540C1 lysogen of this strain and other strains with mutations in ompC repressed OmpF synthesis under conditions which lead to high-level expression of OmpC protein. Strains which are diploid or triploid for ompC show strong inhibition of synthesis of OmpF protein. We conclude that a regulatory element located upstream from the ompC coding sequence inhibits translation of OmpF protein under conditions which favor OmpC expression. Since ompF is known to repress transcription of ompC, we propose that these two genes constitute a closed regulatory loop which acts to amplify regulatory signals which control expression of these proteins.

ACESSO AO ARTIGO

Documentos Relacionados