Regulation of Renal Cortex Ammoniagenesis I. STIMULATION OF RENAL CORTEX AMMONIAGENESIS IN VITRO BY PLASMA ISOLATED FROM ACUTELY ACIDOTIC RATS
AUTOR(ES)
Alleyne, George A. O.
RESUMO
We studied the acute renal metabolic response in rats made acidotic by a single oral dose of ammonium chloride. Cortical slices from acutely (2-h) acidotic rats utilized more glutamine and produced more ammonia and glucose from glutamine than slices from normal animals. When cortical slices from normal rats were pretreated in vitro with plasma isolated from acutely acidotic rats, they achieved similar increases in glutamine utilization, ammonia formation, and gluconeogenesis from glutamine. We did not observe such stimulation in normal cortical slices pretreated in a low pH-low bicarbonate medium. Our data show that a nondialysable factor is present in plasma from acutely acidotic rats that may be responsible for the early increase in the urinary ammonia observed in such animals.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=301445Documentos Relacionados
- Renal metabolic response to acid base changes: I. Enzymatic control of ammoniagenesis in the rat
- pH regulation in single CA1 neurons acutely isolated from the hippocampi of immature and mature rats.
- Stimulation of transcription in vitro by binding sites for nuclear factor I.
- Functional role of thromboxane production by acutely rejecting renal allografts in rats.
- In vitro stimulation of phosphate uptake in isolated chick renal cells by 1,25-dihydroxycholecalciferol.