Regulation of Subunit Synthesis of Escherichia coli RNA Polymerase

AUTOR(ES)
RESUMO

λcI857s7drifd18 phage is a defective, specialized transducing phage carrying the RNA polymerase mutation rifd, a dominant rifampicin-resistant allele. This phage DNA directs in vitro synthesis of the β subunit of RNA polymerase in a DNA-dependent protein-synthesizing system. Prophage induction in cells that are lysogenic for this phage increases the rate of β-subunit synthesis in vivo by at most 2-fold. No increase in subunit synthesis above the haploid level is observed in cells diploid for the β gene.

Documentos Relacionados