Regulation of Telomere Length and Suppression of Genomic Instability in Human Somatic Cells by Ku86
AUTOR(ES)
Myung, Kyungjae
FONTE
American Society for Microbiology
RESUMO
Ku86 plays a key role in nonhomologous end joining in organisms as evolutionarily disparate as bacteria and humans. In eukaryotic cells, Ku86 has also been implicated in the regulation of telomere length although the effect of Ku86 mutations varies considerably between species. Indeed, telomeres either shorten significantly, shorten slightly, remain unchanged, or lengthen significantly in budding yeast, fission yeast, chicken cells, or plants, respectively, that are null for Ku86 expression. Thus, it has been unclear which model system is most relevant for humans. We demonstrate here that the functional inactivation of even a single allele of Ku86 in human somatic cells results in profound telomere loss, which is accompanied by an increase in chromosomal fusions, translocations, and genomic instability. Together, these experiments demonstrate that Ku86, separate from its role in nonhomologous end joining, performs the additional function in human somatic cells of suppressing genomic instability through the regulation of telomere length.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=416406Documentos Relacionados
- Ku86 is essential in human somatic cells
- Ku86 preserves chromatin integrity in cells adapted to high NaCl
- Deletion of Ku86 causes early onset of senescence in mice
- Mammalian Ku86 mediates chromosomal fusions and apoptosis caused by critically short telomeres
- Mammalian Ku86 protein prevents telomeric fusions independently of the length of TTAGGG repeats and the G-strand overhang