Regulation of Thymidine Metabolism in Escherichia coli K-12: Optimal Conditions for the Assay of 1,5-Phosphodeoxyribomutase in Ultrasonic Extracts
AUTOR(ES)
Bonney, Robert J.
RESUMO
Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10−3m thymidine, phosphodeoxyribomutase is induced three- to four-fold. Cell pellets may be frozen for 24 hr before sonic disruption without loss of enzyme activity. The assay is highly reproducible.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=248139Documentos Relacionados
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