Relation between the adenylylation state of glutamine synthetase and the expression of other genes involved in nitrogen metabolism.
AUTOR(ES)
Goldberg, R B
RESUMO
We have partially characterized the biochemical parameters of glutamine synthetase from Klebsiella pneumoniae and have shown that the differential affinity of adenylylated and unadenylylated glutamine synthetase for adenosine diphosphate provides a convenient means of determining the adenylylation state. Using this assay procedure, we examined the relationship between the adenylylation state and the expression of other genes involved in nitrogen assimilation. We observed no correlation between the adenylylation state and the expression of histidase, glutamine synthetase, glutamate synthase, glutamate dehydrogenase, and urease in aerobic cultures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=218311Documentos Relacionados
- Glutamine Synthetase Regulation, Adenylylation State, and Strain Specificity Analyzed by Polyacrylamide Gel Electrophoresis
- Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae.
- Genetic and Physiological Analysis of Germination Efficiency in Maize in Relation to Nitrogen Metabolism Reveals the Importance of Cytosolic Glutamine Synthetase
- Omega-aminoalkyl agaroses in the resolution of enzymes involved in regulation of glutamine metabolism.
- Overexpression of Cytosolic Glutamine Synthetase. Relation to Nitrogen, Light, and Photorespiration1
