Release of Pituitary Growth Hormone by Prostaglandins and Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate in the Absence of Protein Synthesis

AUTOR(ES)

MacLeod, Robert M.

RESUMO

Effects of prostaglandins on the incorporation of [4,5-3H]leucine into growth hormone and its subsequent release into the incubation medium were studied. Incubation of rat anterior pituitary glands with 10-6 M prostaglandin PGE1 in tissue culture medium 199 for 7 hr caused a 40-300% increase in the release of labeled growth hormone into the incubation medium. PGE1 at 10-8 M increased growth hormone synthesis but not release. At 10-6 M, PGE2 had effects similar to PGE1; PGA1 increased growth hormone synthesis but not release. PGF2α was without effect on either synthesis or release of growth hormone.

Documentos Relacionados

• Synthesis and Release of Cyclic Adenosine 3′:5′-Monophosphate by Ochromonas malhamensis1
• Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and Testosterone
• Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate Effects on Goldfish Behavior and Brain RNA Metabolism
• Renal effects of adenosine 3′,5′-cyclic monophosphate and dibutyryl adenosine 3′,5′-cyclic monophosphate: Evidence for a role for adenosine 3′,5′-cyclic monophosphate in the regulation of proximal tubular sodium reabsorption
• The Lack of Effect of Cyclic Adenosine 3′:5′-Monophosphate on Avena Coleoptile Growth 1