Repeated DNA Sequences in the Heterochromatic Y Chromosome of Adult Drosophila melanogaster

AUTOR(ES)

Perreault, W. J.

RESUMO

DNA isolated from Drosophila melanogaster adults (XX, XY, XXY, XYY) was used in DNA-DNA hybridization experiments on nitrocellulose filters. Filter-bound DNA of “high-heterochromatin” flies (those with one or more Y chromosomes) is more effective in forming hybrid duplexes than is XX DNA. The quantitative difference in hybridization efficiency is due primarily to molecules with a relatively high thermal stability (duplexes that dissociate in the temperature range 80-90°). Hybridization experiments with DNA samples that have been fractionated into reiterated and unique portions show that the majority of the hybrid duplexes formed involve reiterated DNA. A small, but highly specific, interaction of unique DNA sequences has also been detected in our experiments. These data indicate that a class of repeated DNA sequences is associated with the constitutive heterochromatin, specifically with the Y chromosome of D. melanogaster. Evidence is presented that this same class of molecules, or very similar ones, is to be found in other portions of the genome, presumably in X-chromosomal or centromeric heterochromatin.

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