Replacement of animal serum proteins by human albumin for growth and interferon production by Namalva cells.
AUTOR(ES)
Field, A K
RESUMO
Growth and interferon production by human lymphoblastoid cells (Namalva) have been achieved by using medium with a complete substitution of human albumin for fetal bovine serum. Medium containing 0.3% human albumin supported exponential cell growth (minimum doubling time, 20 h) in Bilthoven 10-liter fermentors. Interferon induction with Sendai virus resulted in interferon yields of 0.5 x 10(4) to 1.0 x 10(4) interferon units per 10(6) Namalva cells per ml in RPMI 1640 medium containing 0.15 to 0.3% human albumin. Both cell growth and interferon production in medium containing human albumin were comparable to growth and interferon production in the same medium containing 10 and 5% fetal bovine serum, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=352991Documentos Relacionados
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