Replication-competent picornaviruses with complete genomic RNA 3' noncoding region deletions.

AUTOR(ES)

Todd, S

RESUMO

The genomic RNA 3' noncoding region is believed to be a major cis-acting molecular genetic determinant for regulating picornavirus negative-strand RNA synthesis by promoting replication complex recognition. We report the replication of two picornavirus RNAs harboring complete deletions of the genomic RNA 3' noncoding regions. Our results suggest that while specific 3'-terminal RNA sequences and/or secondary structures may have evolved to promote or regulate negative-strand RNA synthesis, the basic mechanism of replication initiation is not strictly template specific and may rely primarily upon the proximity of newly translated viral replication proteins to the 3' terminus of template RNAs within tight membranous replication complexes.

Documentos Relacionados

• A replication-competent promoter-trap retrovirus.
• Replication-competent human mitochondrial DNA lacking the heavy-strand promoter region.
• Replication-competent chimeric lenti-oncovirus with expanded host cell tropism.
• Construction of replication-competent Herpesvirus saimiri deletion mutants.
• Interferon prevents formation of replication-competent hepatitis B virus RNA-containing nucleocapsids