Replication of Canine Herpesvirus: I. Synthesis of Viral Deoxyribonucleic Acid

AUTOR(ES)

Aurelian, Laure

RESUMO

This paper reports the results of two series of experiments. The first series indicated that deoxyribonucleic acid (DNA) extracted from partially purified canine herpesvirus virions is characterized by a high guanine plus cytosine molar base ratio (65 to 67 mole%), similar to the DNA of herpes simplex virus. In the second series of experiments it was estimated, on the basis of uptake of tritiated thymidine, that in dog kidney cells canine herpesvirus-DNA synthesis starts at 4 hr and continues until 16 hr after infection. Treatment of infected cells with puromycin during the first 4 hr of infection blocks the onset of viral DNA synthesis, whereas, after this time the uptake of thymidine is unaffected.

Documentos Relacionados

• Replication of Canine Herpesvirus: II. Virus Development and Release in Infected Dog Kidney Cells
• Polyamines in the Synthesis of Bacteriophage Deoxyribonucleic Acid. I. Lack of Dependence of Polyamine Synthesis on Bacteriophage Deoxyribonucleic Acid Synthesis
• Deoxyribonucleic Acid Synthesis in Bacteriophage SPO1-Infected Bacillus subtilis I. Bacteriophage Deoxyribonucleic Acid Synthesis and Fate of Host Deoxyribonucleic Acid in Normal and Polymerase-Deficient Strains
• Regulation of Herpesvirus Macromolecular Synthesis I. Cascade Regulation of the Synthesis of Three Groups of Viral Proteins 1
• Inhibition of Herpesvirus Replication and Herpesvirus-Induced Deoxyribonucleic Acid Polymerase by Phosphonoformate†