Replication of the Human Hepatitis Delta Virus Genome Is Initiated in Mouse Hepatocytes following Intravenous Injection of Naked DNA or RNA Sequences

AUTOR(ES)

Chang, Jinhong

FONTE

American Society for Microbiology

RESUMO

As early as 5 days after DNA copies of the hepatitis delta virus (HDV) genome or even in vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the hydrodynamics-based transfection procedure of F. Liu et al. (Gene Ther. 6:1258–1266, 1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of protein, and immunostaining of liver sections what were considered typical features of HDV genome replication. This transfection strategy should have valuable applications for in vivo studies of HDV replication and pathogenesis and may also be useful for studies of other hepatotropic viruses.

Documentos Relacionados

• Replication of hepatitis delta virus RNA in mice after intramuscular injection of plasmid DNA.
• Replication of human hepatitis delta virus in primary cultures of woodchuck hepatocytes.
• Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen.
• Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus.
• Hepatitis delta virus genome replication: a polyadenylated mRNA for delta antigen.