Replication of the R Factor Rts1 in Proteus mirabilis

AUTOR(ES)

Terawaki, Yoshiro

RESUMO

The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and supplemented M9 minimal medium at 30 C. The chromosomal DNA content per cell varied over a threefold range in the different growth media. In agreement with previous genetic observations, the replication of Rts1 was found to be temperature-sensitive and Rts1 DNA was diluted from the cells during exponential growth at 42 C. 14N-15N medium transfer experiments have shown that individual copies of Rts1 are selected at random for replication during the duplication of the multicopy episome pool.

Documentos Relacionados

• Replication of the R Factor Rts1 in Proteus mirabilis
• Effects of Inhibition and Restoration of Protein Synthesis on the Replication of the R Factor Rts1 in Proteus mirabilis
• The Thermosensitive Lesion in the Replication of the Drug Resistance Factor, Rts1
• Molecular Nature of the Deoxyribonucleic Acid of a Thermosensitive R Factor, Rts1
• Positive and Negative Control of R-Factor Replication in Proteus mirabilis