Repression of c-fos transcription is mediated through p67SRF bound to the SRE.
AUTOR(ES)
Shaw, P E
RESUMO
Human c-fos expression is subject to tight transcriptional control. The gene is expressed at a high, constitutive level in some cell types and at a very low, but rapidly inducible level in many others. Induction of transcription by serum growth factors is mediated by the serum response element (SRE) to which at least two transcription factors, p67SRF and p62, bind. In this paper it is demonstrated that the low basal level of transcription is mediated through p67SRF bound to the SRE and that high, constitutive expression is observed when binding is prohibited. In this situation, an AP-1 consensus binding site adjacent to the SRE permits transactivation of the gene. Thus three levels of c-fos expression, induced, repressed and constitutive, appear to be determined by occupation of the SRE by p67SRF and its interaction with other proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=401258Documentos Relacionados
- Phosphorylation-dependent formation of a quaternary complex at the c-fos SRE.
- Casein kinase II induces c-fos expression via the serum response element pathway and p67SRF phosphorylation in living fibroblasts.
- DNA-bound transcription factor complexes analysed by mass-spectrometry: binding of novel proteins to the human c-fos SRE and related sequences
- Repression of the basal c-fos promoter by wild-type p53.
- Synergism in ternary complex formation between the dimeric glycoprotein p67SRF, polypeptide p62TCF and the c-fos serum response element.