Residual Thymidine Incorporation in the Presence of Chloramphenicol in Synchronous Cultures of Escherichia coli B/r

AUTOR(ES)
RESUMO

Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 μg/ml) and [14C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an estimate of the length of the D period rather than the time of initiation of chromosome replication.

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