Residue Aspartate-147 from the Third Transmembrane Region of Na+/H+ Antiporter NhaB of Vibrio alginolyticus Plays a Role in Its Activity

AUTOR(ES)

Nakamura, Tatsunosuke

FONTE

American Society for Microbiology

RESUMO

NhaB is a bacterial Na+/H+ antiporter with unique topology. The pH dependence of NhaB from Vibrio alginolyticus differs from that of the Escherichia coli NhaB homolog. Replacement of Asp-147 with Glu made high H+ concentrations a requirement for the NhaB activity. Replacement of Asp-147 with neutral amino acids inactivated NhaB.

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