ResistÃncia das leveduras Kluyveromyces marxianus e Saccharomyces cerevisiae ao fungicida Benomyl

AUTOR(ES)

Yasodhara Silva Lacerda

DATA DE PUBLICAÇÃO

2007

RESUMO

Investigations on the toxic compound-resistant phenotypes by microrganisms are regarded as relevant within the bio-technological, clinic and farming fields. In this work, we aimed to identify the co-relation between the resistance levels of yeast cells to the fungicide Benomyl and the presence of two yeast genes already related to have any influence in this response. A wild type strain of K. marxianus and several industrial strains of S. cerevisiae were surveyed for the inter and intraspecific va riance of such resistance. The molecular approach included the detection of certain alleles of both TUB2 and ICT1 genes. The TUB2 gene encodes the β-tubulin protein involved in cytoskeleton formation. Allele variation are involved in Benomyl resistance phenotype. The ICT1 gene is part of the MDR (Multi-drug Resistance) complex. Its biological function still unknown, although its inactivation causes drug sensitivity. The analysis on the cell growth kinetics, allied to the gene amplification profiles, suggested an intricate cell defense mechanism to this kind of drugs

ASSUNTO(S)

benomyl resistÃncia celular cell resistance yeasts genetica leveduras benomylÂ

Documentos Relacionados

• CorrelaÃÃo entre microestrutura, resistÃncia mecÃnica e resistÃncia à corrosÃo da liga ZA27.
• Cotidiano e resistÃncia nas Ãltimas dÃcadas da escravidÃo, Olinda, 1871-1888
• InfluÃncia da extensÃo e tipo de retentor intra-radicular na deformacÃo e resistÃncia à fratura de dentes tratados endodonticamente
• O GRAB e a resistÃncia homoerÃtica no CearÃ
• InfluÃncia do Teor de Mo na ResistÃncia à CorrosÃo e FormaÃÃo de Fases em Ligas Modelos Fe-Cr-Mo