Resposta imune celular em culturas de células do sistema nervoso central de rato infectados com Neospora Caninum.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Neosporosis is a disease caused by Neospora caninum, an intracellular parasite that infects a wide range of animals, causes abortion in cattle, and neurologic disease in dogs. To study the pathogenic effects of this parasite, especially its correlation with the immunological system, we described a model of infection in vitro, using rat glial cells. Glial cells are responsible for homeostasis, nutrition, as well as by the immune response. Interactions between neurons and glia are essential to the development and function of the central neurvous system (CNS). The model was well established, concerning the dose and time for infection and the expression of cytokines; infected glial cultures show modification in morphology, characterized by the large cell bodies and intermediate filament reorganization. Astrocytes infected for 24 and 72h release TNF-a and NO, as well as, high levels of IL-10 and have increased lactate dehydrogenase activity. Infected glial cells, were also studied on its respiratory metabolism, by polarography, in order to evaluate the consumption of oxygen but, no changes were seen, when compared with control. Mixed glial cultures with about 86% of astrocytes and 12% of microglia, were analyzed for the immune response, when infected by the N. caninum, theses cultures released TNF-a and NO, as well as high levels of IL-10 and IL-6. In both kinds of cultures, IFN-g was not detected, probably in function of the high levels of IL-10. All models are adequate to study the effects of neosporosis in the CNS where infected glial cells with N. caninum show a Th2 type response.

ASSUNTO(S)

neospora caninum central nervous system immune response resposta imune neuroglia astrocyte neospora caninum imunologia neuroglia sistema nervoso central astrócitos

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