Restricted Replication of Frog Virus 3 in Selected Variants of BHK Cells
AUTOR(ES)
Vilaginès, R.
RESUMO
Synthesis and maturation of frog virus 3 deoxyribonucleic acid (DNA) in BHK cells and selected variants, in chick fibroblasts, and in minnow cells were compared. Wide ranges in rates of DNA synthesis and assembly of virions were found. At least three variants of BHK cells can be obtained: (i) fully permissive, characterized by rapid DNA synthesis and assembly to give a high yield of infective virus; (ii) semipermissive, in which viral DNA is synthesized slowly for extended periods of time, and the yield of infective virus is poor; and (iii) nonpermissive, in which virus adsorbs to cells and arrests host functions but viral DNA is not synthesized. Kinetics of appearance of virions and procedures for their separation from cell extracts are described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376123Documentos Relacionados
- Frog Virus 3 Replication: Analysis of Structural and Nonstructural Polypeptides in Infected BHK Cells by Acidic and Basic Two-Dimensional Gel Electrophoresis
- Macromolecular synthesis in cells infected by frog virus 3. VI. Frog virus 3 replication is dependent on the cell nucleus.
- Frog Virus 3 Replication: Induction and Intracellular Distribution of Polypeptides in Infected Cells
- Phosphonoacetic Acid Inhibition of Frog Virus 3 Replication
- Restricted Replication of Vesicular Stomatitis Virus in Human Lymphoblastoid Cells