Retention of a recombinant GFP protein expressed by the yellow fever 17D virus in the E/NS1 intergenic region in the endoplasmic reticulum
AUTOR(ES)
Trindade, Gisela Freitas, Santana, Marlon Gilsepp Veloso de, Santos, Juliana Ribeiro dos, Galler, Ricardo, Bonaldo, Myrna Cristina
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2012-03
RESUMO
The flaviviral envelope proteins, E protein and precursor membrane protein, are mainly associated with the endoplasmic reticulum (ER) through two transmembrane (TM) domains that are exposed to the luminal face of this compartment. Their retention is associated with the viral assembly process. ER-retrieval motifs were mapped at the carboxy terminus of these envelope proteins. A recombinant yellow fever (YF) 17D virus expressing the reporter green fluorescent protein (GFP) with the stem-anchor (SA) region of E protein fused to its carboxy terminus was subjected to distinct genetic mutations in the SA sequence to investigate their effect on ER retention. Initially, we introduced progressive deletions of the stem elements (H1, CS and H2). In a second set of mutants, the effect of a length increase for the first TM anchor region was evaluated either by replacing it with the longer TM of human LAMP-1 or by the insertion of the VALLLVA sequence into its carboxy terminus. We did not detect any effect on the GFP localisation in the cell, which remained associated with the ER. Further studies should be undertaken to elucidate the causes of the ER retention of recombinant proteins expressed at the intergenic E/NS1 region of the YF 17D virus polyprotein.
Documentos Relacionados
- Clonagem and expression of protein EGFP in the region intergenic E/NS1 of cepa vaccine 17D of the virus of the Yellow Fever.
- Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization
- Is a dose of 17D vaccine in the current context of Yellow Fever enough?
- The early use of yellow fever virus strain 17D for vaccine production in Brazil - a review
- Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural protein NS1.