Ribosomal DNA and Stellate gene copy number variation on the Y chromosome of Drosophila melanogaster.
AUTOR(ES)
Lyckegaard, E M
RESUMO
Multigene families on the Y chromosome face an unusual array of evolutionary forces. Both ribosomal DNA and Stellate, the two families examined here, have multiple copies of similar sequences on the X and Y chromosomes. Although the rate of sequence divergence on the Y chromosome depends on rates of mutation, gene conversion and exchange with the X chromosome, as well as purifying selection, the regulation of gene copy number may also depend on other pleiotropic functions, such as maintenance of chromosome pairing. Gene copy numbers were estimated for a series of 34 Y chromosome replacement lines using densitometric measurements of slot blots of genomic DNA from adult Drosophila melanogaster. Scans of autoradiographs of the same blots probed with the cloned alcohol dehydrogenase gene, a single copy gene, served as internal standards. Copy numbers span a 6-fold range for ribosomal DNA and a 3-fold range for Stellate DNA. Despite this magnitude of variation, there was no association between copy number and segregation variation of the sex chromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286821Documentos Relacionados
- Structural genes on the Y chromosome of Drosophila melanogaster.
- Dosage regulation of ribosomal DNA in Drosophila melanogaster.
- Evolution of the Ribosomal DNA Spacers of Drosophila melanogaster : Different Patterns of Variation on X and Y Chromosomes
- Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
- Molecular analysis of the yellow gene (y) region of Drosophila melanogaster.
