Ribosome-dependent conversion of polyA-containing heterogenous nuclear RNA into smaller RNA molecules.
AUTOR(ES)
Grozdanovic, J
RESUMO
The polyA-containing heterogenous nuclear RNA fraction separated from total rat liver nRNA by gel filtration on Sepharose 4B followed by affinity chromatography on polyU-Sepharose and containing predominantly the 45S components becomes enzymatically bound to homologous 80S ribosomes and polyribosomes at 0 degree C. If 80S ribosomes or polyribosomes with bound poly-a-containing HnRNA are subjected to a further incubation at 37 degree C, the original 45S RNA is gradually converted into smaller RNA species of 10- 35S which remain bound to the particle. This ribosome-dependent cleavage of larger HnRNA species into smaller RNA molecules may represent the ultimate step of mRNA maturation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343469Documentos Relacionados
- A ribosome-dependent GTPase from yeast distinct from elongation factor 2.
- Comparison of polysomal and nuclear poly(A)-containing RNA populations from normal rat liver and Novikoff hepatoma.
- The relA/spoT-Homologous Gene in Streptomyces coelicolor Encodes Both Ribosome-Dependent (p)ppGppSynthesizing and -Degrading Activities
- Requirement of Proteins S5 and S9 from 30S Subunits for the Ribosome-Dependent GTPase Activity of Elongation Factor G
- Isolation and characterization of poly(A)-containing intranuclear polyoma-specific "giant" RNA'S.