RNA complementary to herpes simplex virus type 1 ICP0 gene demonstrated in neurons of human trigeminal ganglia.
AUTOR(ES)
Gordon, Y J
RESUMO
Recent studies with mice have demonstrated abundant RNA transcripts which are complementary (antisense) to the herpes alpha gene ICP0 in latently infected ganglia. We investigated the situation in unselected human trigeminal ganglia. Strand-specific 2.7-kilobase herpes simplex virus type 1 (HSV-1) ICP0 RNA probes were prepared, and their sense was determined in productively infected cells. Although in situ hybridization demonstrated ICP0 antisense RNA transcripts in the nuclei of neurons in 46% of the ganglia, ICP0 messenger RNA was not found in any of the ganglia. We conclude that HSV-1 antisense ICP0 RNA is present in humans during ganglionic latency.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253243Documentos Relacionados
- Latent herpes simplex virus type 1 transcription in human trigeminal ganglia.
- Monoclonal antibodies suppress replication of herpes simplex virus type 1 in trigeminal ganglia.
- Neurons Differentially Activate the Herpes Simplex Virus Type 1 Immediate-Early Gene ICP0 and ICP27 Promoters in Transgenic Mice
- Detection and preliminary characterization of herpes simplex virus type 1 transcripts in latently infected human trigeminal ganglia.
- Herpes Simplex Virus Type 1 ICP0 Protein Does Not Accumulate in the Nucleus of Primary Neurons in Culture