RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family
AUTOR(ES)
Bies-Etheve, Natacha
FONTE
Nature Publishing Group
RESUMO
Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxy-terminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2711833Documentos Relacionados
- RNA-directed DNA methylation in Arabidopsis
- Modulating HIV-1 replication by RNA interference directed against human transcription elongation factor SPT5
- Induction of RNA-directed DNA methylation upon decondensation of constitutive heterochromatin
- DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs
- Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato.
