RNA polymerase as a repressor of transcription in the hut(P) region of mutant Klebsiella aerogenes histidine utilization operons.
AUTOR(ES)
Nieuwkoop, A J
RESUMO
Mutants of Klebsiella aerogenes able to express the hutUH operon in the absence of positive effectors were isolated and characterized. These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely. These mutations also affect another, oppositely oriented promoter in this region, PC. Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site. Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211555Documentos Relacionados
- Bidirectional promoter in the hut(P) region of the histidine utilization (hut) operons from Klebsiella aerogenes.
- Gene order of the histidine utilization (hut) operons in Klebsiella aerogenes.
- In vitro transcription of the histidine utilization (hutUH) operon from Klebsiella aerogenes.
- Cloning of the Klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in Salmonella typhimurium.
- Identification of important chemical groups of the hut mRNA for HutP interactions that regulate the hut operon in Bacillus subtilis