RNA replication by Q beta replicase: a working model.
AUTOR(ES)
Brown, D
RESUMO
Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Q beta replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism of RNA replication employed by the four-subunit replicase. Replication inhibition by site I- and site II-specific ligands defined two subsets of replicatable RNAs. When provided with appropriate 3' ends, ligands to either site served as replication templates. UV crosslinking experiments revealed that site I is associated with the S1 subunit, site II with elongation factor Tu, and polymerization with the viral subunit of the holoenzyme. These results provide the framework for a three site model describing template recognition and product strand initiation by Q beta replicase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38096Documentos Relacionados
- In vitro recombination and terminal elongation of RNA by Q beta replicase.
- Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.
- Minimal requirements for template recognition by bacteriophage Qβ replicase: Approach to general RNA-dependent RNA synthesis
- Q beta replicase containing a Bacillus stearothermophilus elongation factor.
- Replication of RNA viruses, IV. Initiation of RNA synthesis by the q-beta RNA polymerase.