Role for the Silencing Protein Dot1 in Meiotic Checkpoint Control
AUTOR(ES)
San-Segundo, Pedro A.
FONTE
The American Society for Cell Biology
RESUMO
During the meiotic cell cycle, a surveillance mechanism called the “pachytene checkpoint” ensures proper chromosome segregation by preventing meiotic progression when recombination and chromosome synapsis are defective. The silencing protein Dot1 (also known as Pch1) is required for checkpoint-mediated pachytene arrest of the zip1 and dmc1 mutants of Saccharomyces cerevisiae. In the absence of DOT1, the zip1 and dmc1 mutants inappropriately progress through meiosis, generating inviable meiotic products. Other components of the pachytene checkpoint include the nucleolar protein Pch2 and the heterochromatin component Sir2. In dot1, disruption of the checkpoint correlates with the loss of concentration of Pch2 and Sir2 in the nucleolus. In addition to its checkpoint function, Dot1 blocks the repair of meiotic double-strand breaks by a Rad54-dependent pathway of recombination between sister chromatids. In vegetative cells, mutation of DOT1 results in delocalization of Sir3 from telomeres, accounting for the impaired telomeric silencing in dot1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=15018Documentos Relacionados
- Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association
- A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint
- Meiotic DNA replication checkpoint control in fission yeast
- TopBP1 and ATR Colocalization at Meiotic Chromosomes: Role of TopBP1/Cut5 in the Meiotic Recombination Checkpoint
- An essential role of DmRad51/SpnA in DNA repair and meiotic checkpoint control