Role of 5S RNA in the Functions of 50S Ribosomal Subunits*

AUTOR(ES)

Erdmann, V. A.

RESUMO

50S ribosomal subunits from Bacillus stearothermophilus can be reconstituted from their dissociated components, namely a 5S RNA-free protein fraction, a 5S RNA-free 23S ribosomal RNA fraction, and purified 5S RNA. The biological activity of reconstituted particles in polypeptide synthesis is dependent on the presence of 5S RNA. In the absence of 5S RNA, particles are produced that have greatly reduced activity in (a) polypeptide synthesis directed by synthetic, as well as natural, messenger RNA, (b) peptidyl transferase assay, (c) [3H]UAA binding dependent on peptide chain termination factor R1, (d) G factor-dependent [3H]GTP binding, and (e) codon-directed tRNA binding assayed in the presence of 30S subunits. Thus, 5S RNA is an essential 50S ribosomal component.

Documentos Relacionados

• Chemical reactivity of E. coli 5S RNA in situ in the 50S ribosomal subunit.
• Reconstitution of biologically active 50S ribosomal subunits with artificial 5S RNA molecules carrying disturbances in the base pairing within the molecular stalk.
• Binding of specific sRNA to 30S ribosomal subunits: effect of 50S ribosomal subunits.
• Role of 5S RNA in assembly and function of the 50S subunit from Escherichia coli.
• Structure and Function of Bacterial Ribosomes, XI. Dependence of 50S Ribosomal Assembly on Simultaneous Assembly of 30S Subunits*