Role of gene 2 in bacteriophage T7 DNA synthesis.
AUTOR(ES)
Center, M S
RESUMO
Studies have been carried out to elucidate the in vivo function of gene 2 in T7 DNA synthesis. In gene 2-infected cells the rate of incorporation of (3-H)thymidine into acid-insoluble material is about 60% that of cells infected with T7 wild type. Gene 2 mutants do not however produce viable phage after infection of the nonpermissive host. In T7 wild type-infected cells, a major portion of the newly alkaline sucrose gradients. The concatemers serve as precursors for the formation of mature T7 DNA as demonstrated in pulse-chase experiments. In similar studies carried out with gene 2-infected cells, concatemers are not detected when the intracellular DNA is analyzed at several different times during the infection process. The DNA made during a gene 2 infection is present as duplex structures with a sedimentation rate close to mature T7 DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354636Documentos Relacionados
- Role of bacteriophage T7 DNA primase in the initiation of DNA strand synthesis.
- The role of bacteriophage T7 gene 2 protein in DNA replication.
- Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.
- Bacteriophage T7 DNA Synthesis in Isolated DNA-Membrane Complexes
- Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro.