Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans.
AUTOR(ES)
Kanamori, K
RESUMO
Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells in which the intracellular NH4+ concentration was 1.4 +/- 0.5 mM, the activity of GDH with a Km of 2.2 mM for NH4+ was found to be severalfold higher than that of glutamate synthase. The result suggests that GDH plays a significant role in the assimilation of NH4+ in N2-fixing B. macerans.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213841Documentos Relacionados
- Ammonia assimilation pathways in nitrogen-fixing Clostridium kluyverii and Clostridium butyricum.
- Quorum Sensing in Nitrogen-Fixing Rhizobia
- Nitrogen-Fixing (Acetylene Redution) Activity and Population of Aerobic Heterotrophic Nitrogen-Fixing Bacteria Associated with Wetland Rice
- Hemoglobin in Frankia, a Nitrogen-Fixing Actinomycete†
- Efficiency factors and ATP/ADP ratios in nitrogen-fixing Bacillus polymyxa and Bacillus azotofixans.
