Role of R loops in recA-independent homologous recombination of bacteriophage lambda.
AUTOR(ES)
Matsumoto, T
RESUMO
We have previously shown that the DNA-dependent RNA polymerase of Escherichia coli can promote homologous recombination of bacteriophage lambda independently of the recA function. To detect this recombination, we jointly infected the cells with a pair of lambda phages in the presence of chloramphenicol, extracted intracellular lambda DNA molecules, packaged them in vitro, and measured the number of resulting recombinant phage particles. We showed that the recombination of DNA molecules takes place in vitro after extraction of DNA from the cells. We fractionated recombinogenic forms of intracellular lambda DNA and showed that they carry RNA. These and other results suggest that the R (RNA) loop structures, generated by transcription within the cells, promote homologous recombination in vitro. We discuss possible mechanisms of this recombination and compare those with the other forms of general recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256503Documentos Relacionados
- Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.
- RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli
- Involvement of DNA-dependent RNA polymerase in a recA-independent pathway of genetic recombination in Escheria coli.
- RecA-independent ectopic transposition in vivo of a bacterial group II intron
- RecA-independent recombination at gamma delta termini and at IS3 producing inverted repetition in F' plasmids.