Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.

AUTOR(ES)

Hendrix, R W

RESUMO

Correct assembly of the heads of bacteriophages lambda and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of lambda, called lambda gt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type lambda, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.

Documentos Relacionados

• Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product).
• Identity of the B56.5 protein, the A-protein, and the groE gene product of Escherichia coli.
• Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis.
• Cloning the spoT gene of Escherichia coli: identification of the spoT gene product.
• Evidence that the two Escherichia coli groE morphogenetic gene products interact in vivo.