Roles of Two Ca2+-binding Domains in Regulation of the Cardiac Na+-Ca2+ Exchanger*

AUTOR(ES)

Ottolia, Michela

FONTE

American Society for Biochemistry and Molecular Biology

RESUMO

We expressed full-length Na+-Ca2+ exchangers (NCXs) with mutations in two Ca2+-binding domains (CBD1 and CBD2) to determine the roles of the CBDs in Ca2+-dependent regulation of NCX. CBD1 has four Ca2+-binding sites, and mutation of residues Asp421 and Glu451, which primarily coordinate Ca2+ at sites 1 and 2, had little effect on regulation of NCX by Ca2+. In contrast, mutations at residues Glu385, Asp446, Asp447, and Asp500, which coordinate Ca2+ at sites 3 and 4 of CBD1, resulted in a drastic decrease in the apparent affinity of peak exchange current for regulatory Ca2+. Another mutant, M7, with 7 key residues of CBD1 replaced, showed a further decrease in apparent Ca2+ affinity but retained regulation, confirming a contribution of CBD2 to Ca2+ regulation. Addition of the mutation K585E (located in CBD2) into the M7 background induced a marked increase in Ca2+ affinity for both steady-state and peak currents. Also, we have shown previously that the CBD2 mutations E516L and E683V have no Ca2+-dependent regulation. We now demonstrate that introduction of a positive charge at these locations rescues Ca2+-dependent regulation. Finally, our data demonstrate that deletion of the unstructured loops between β-strands F and G of both CBDs does not alter the regulation of the exchanger by Ca2+, indicating that these segments are not important in regulation. Thus, CBD1 and CBD2 have distinct roles in Ca2+-dependent regulation of NCX. CBD1 determines the affinity of NCX for regulatory Ca2+, although CBD2 is also necessary for Ca2+-dependent regulation.

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