Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells.
AUTOR(ES)
Skaliter, R
RESUMO
Whole-cell extracts of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex DNA molecules. The products of the reaction are longer than monomer unit length and are the result of semiconservative DNA replication by the following criteria: (i) resistance to DpnI and susceptibility to MboI restriction enzymes, (ii) shift in density on a CsCl gradient of the products synthesized in the presence of bromo-dUTP to a position on the gradient consistent with those of molecules composed mainly of one parental DNA strand and one newly synthesized DNA strand, and (iii) the appearance in the electron microscope of molecules consisting of duplex circles with multiunit linear appendages, a characteristic of a rolling circle mode of DNA replication. The reaction requires ATP and is dependent on herpes simplex virus type 1-encoded DNA polymerase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=189921Documentos Relacionados
- Suppression of apoptotic DNA fragmentation in herpes simplex virus type 1-infected cells.
- Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
- Replication of herpes simplex virus type I DNA in permeabilized infected cells.
- Virus-specific glycoproteins associated with the nuclear fraction of herpes simplex virus type 1-infected cells.
- Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.