Rotaviruses electropherotyes in diarrhoeal faeces and after isolation in MA104 cell cultures / Perfil eletroforético de rotavírus em amostras fecais diarréicas e após isolamento em cultura de células da linhagem MA104
AUTOR(ES)
Thais Lourenço Ferreira
DATA DE PUBLICAÇÃO
2006
RESUMO
In the present work, we have analyzed the electrophoretic profile of 15 samples of rotavírus, 11 of calves, 02 of pigs and 02 of children, that have been cultivated on MA,104 cell up to the sixth passage. The polyacrilamide gel electrophoresis(PAGE) was used to confirm the viral isolation. The aim of the work was to compare the electropherotic profile of fecal samples before and after isolation in MA104 cells. The samples from pigs did not reveal apparent changes in bands migration. However, an additional segment, betweem segments 5 and 6 was detected, in one fecal sample and after isolation. Furthermore, one child sample showed differences in the electrophoretic mobility and also an additional segment was detected between segments 5 - 6 in one sample from a calf. The results emphasize the importance of PAGE as a technique to screen samples that could be analyzed posteriorly by more specific molecular techniques. The changes in the electrophoretic profiles of genomic segments of viral RNA might be suggestive of changes in the antigenic properties, besides the fact that changes that occurred in vitro may also happen in vivo.
ASSUNTO(S)
perfil eletroferótipo cultivo celular page rotavirus electropherotic profile rna cell culture rna page rotavirus
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