Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts
AUTOR(ES)
Mitsuzawa, Hiroshi
FONTE
Oxford University Press
RESUMO
Rpb4–Rpb7, a dissociable subcomplex of RNA polymerase II (pol II), is required for transcription initiation. To understand the role of Rpb7 in transcription initiation or other processes in transcription, we carried out a two-hybrid screen for proteins that interact with Rpb7 of the fission yeast Schizosaccharomyces pombe. The screen identified the S.pombe homolog of the Saccharomyces cerevisiae Nrd1, an RNA-binding protein implicated in 3′ end formation of small nucleolar and small nuclear RNAs transcribed by pol II. The S.pombe protein, named Seb1 for seven binding, was essential for cell viability, and bound directly to Rpb7 in vitro. Saccharomyces cerevisiae Rpb7 also interacted with Nrd1, indicating that the interaction is conserved in evolution. Glu166 and/or Asp167 of S.pombe Rpb7, residues near the C-terminus of the 172 amino acid protein, were found to be important for its interaction with Seb1. Our results suggest that Rpb7 may function to anchor a processing factor to the pol II apparatus, thereby coupling RNA processing to transcription. The role for Rpb7 is consistent with its location in the pol II complex determined by recent structural studies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=169969Documentos Relacionados
- RNA-binding site in T7 RNA polymerase
- Rpb7 Can Interact with RNA Polymerase II and Support Transcription during Some Stresses Independently of Rpb4
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation
- RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing
- A new double-stranded RNA-binding protein that interacts with PKR