S1 sensitive sites in adenovirus DNA.

AUTOR(ES)

Goding, C R

RESUMO

S1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with an inverted repeat region in the DNA sequence. In addition an S1 sensitive site was dictated by a potential cruciform structure in the region of the Ad 2 major late promoter. In contrast to the expected cleavage site at the loop of the cruciform, cleavage occurred at the base of the stem in the region of the TATA box. All three S1 sensitive sites identified were more sensitive to S1 than the endogenous sites in the parent plasmids.

Documentos Relacionados

• Clusters of S1 nuclease-hypersensitive sites induced in vivo by DNA damage.
• Sequence effect on alkali-sensitive sites in UV-irradiated SV40 DNA.
• S1 nuclease hypersensitive sites in an oligopurine/oligopyrimidine DNA from the t(10;14) breakpoint cluster region.
• Escherichia coli ribosomal protein S1 has two polynucleotide binding sites.
• Use of a novel S1 nuclease RNA-mapping technique to measure efficiency of transcription termination on polyomavirus DNA.