Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae.

AUTOR(ES)

Baril, C

RESUMO

Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.

Documentos Relacionados

• Efficacy of ciprofloxacin against Leptospira interrogans serogroup icterohaemorrhagiae.
• Unique organization of Leptospira interrogans rRNA genes.
• Nucleotide sequence of a 16S rRNA gene for Leptospira interrogans serovar canicola strain Moulton.
• Isolation and characterization of the 5S rRNA gene of Leptospira interrogans.
• Effect of mycolic acid and its derivatives on the growth of Leptospira icterohaemorrhagiae.