Screening procedure for complementation-dependent mutants of vesicular stomatitis virus.

AUTOR(ES)

Rettenmier, C W

RESUMO

To isolate new types of vesicular stomatitis virus (VSV) mutants, a four-stage screen was developed which identifies and characterizes mutants capable of complementing the defect in the VSV temperature-sensitive mutant tsG11. Two types of mutants of VSV, Indiana serotype, have been found by using the screen; they are new temperature-sensitive mutants which are, of necessity, not in complementation group I and mutants which do not produce plaques under conditions of single infection at 31 C (the normal permissive temperature) and are, therefore, called complementation-dependent mutants. The newly isolated, temperature-sensitive mutants fall into three complementation groups, two of which are congruent with known complementation groups; the newly identified group extends to six the number of complementation groups of VSV Indiana. The nature of the complementation-dependent mutants has not been established, but one was shown to not contain a significant deletion in its nucleic acid.

Documentos Relacionados

• Nongenetic complementation of group V temperature-sensitive mutants of vesicular stomatitis virus by UV-irradiated virus.
• Sequence alterations in temperature-sensitive M-protein mutants (complementation group III) of vesicular stomatitis virus.
• Primary In Vivo Transcription of Vesicular Stomatitis Virus and Temperature-Sensitive Mutants of Five Vesicular Stomatitis Virus Complementation Groups
• Virion trascriptase activity differences in host range mutants of vesicular stomatitis virus.
• Host function-dependent induction of defective interfering particles of vesicular stomatitis virus.