Second-site revertants of the P1 copN22 copy mutant.
AUTOR(ES)
Froehlich, B J
RESUMO
Miniplasmids with the P1 copN22 mutation have a copy number about seven times that of the wild type. Selection for reduced copy number from this plasmid led to the isolation of second-site pseudorevertants, called poc mutants. DNA sequence analysis showed that all six independent poc mutants have a single base change in the same codon of the repA gene. This implicates the amino acid at this location, either directly or indirectly, in interactions important for copy number control.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208922Documentos Relacionados
- Second-Site Revertants of Escherichia Coli Trp Repressor Mutants
- Second-site suppressors of the bacteriophage P1 virs mutant reveal the interdependence of the c4, icd, and ant genes in the P1 immI operon.
- Genetic analysis of second-site revertants of bacteriophage lambda integrase mutants.
- Effects of second-site mutations on dominant interference by a human immunodeficiency virus type 1 envelope glycoprotein mutant.
- Debilitation of plant potyvirus infectivity by P1 proteinase-inactivating mutations and restoration by second-site modifications.
