Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules.
AUTOR(ES)
Zhang, T Y
RESUMO
A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 megabase pairs to over 10 megabase pairs in size. This improved resolution is obtained with dramatically shorter run times. Thus SPFG appears to circumvent a number of the key limitations in previous PFG protocols.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=333856Documentos Relacionados
- Pulsed-field electrophoresis: application of a computer model to the separation of large DNA molecules.
- Separation of chromosomal DNA molecules from C.albicans by pulsed field gel electrophoresis.
- Transverse gradient gel electrophoresis: a gel system for resolving complex mixtures of lariat and linear RNA molecules.
- DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology.
- A model for the separation of large DNA molecules by crossed field gel electrophoresis.