Seleção de linhagens de Saccharomyces cerevisiae potencializadas pelo fator Killer, H2S- e o carater floculante. / Improvement of a saccharomyces cerevisiae strain by the characters: "Killer" skills, flocculation capacity and lack in productinof H2S-.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Flocculative and "killer" skills and lack in production of H2S are desirable characteristics of the ethanolic fermentative yeasts. Seven selected strains of Saccharomyces cerevisiae with some of these characteristics were evaluated for confirmation of these habilities and their genetic characterization was undertaken by eletrophoretic kariotyping. The strain ATCC 26602 had flocculant hability and the strain K1 was H2S - and "killer". The strains were selected for protoplast fusion aiming to obtain a stable fusion strain with these desirable technologyc characteristics. The selection of the hybrid strains were based on natural characters and have shown 1291 hybrids (frequency of 1,5%) in the medium for the isolation of the fusionants (protoplasts). The protoplast stability were monitored by three continuous growth in the YEPD liquid midium and the stable fusion products were not obtained.

ASSUNTO(S)

yeast fusão de protoplasto levedura floculação flocculation protoplast fusion

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