Selection-expression plasmid vectors for use in genetic transformation of higher plants.
AUTOR(ES)
Velten, J
RESUMO
Plasmid vectors containing both a selectable marker for plant transformation (kanamycin resistance) and a second, directly adjacent, divergent promoter for the transcription of inserted DNA fragments have been constructed. These vectors make use of a small (479 bp) dual-promoter DNA fragment, originally isolated from the T-DNA of Agrobacterium tumefaciens, fused to the neomycin phosphotransferase gene of Tn5. Several unique restriction enzyme cleavage sites, as well as a polyadenylation signal sequence, have been introduced downstream of the open promoter, allowing simple insertional cloning of DNA fragments to be expressed in plants. To test the vectors, the coding region for the chloramphenicol acetyltransferase gene (CAT) from Tn9 was inserted, and the resulting plasmids introduced into tobacco cells. Transformed calli, selected only for Km resistance, contained, in every case tested, both NPTII and CAT activities.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=322017Documentos Relacionados
- Stable transformation of plastids in higher plants.
- Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.
- Stability and expression of bacterial genes in replicating geminivirus vectors in plants.
- Sugar sensing in higher plants.
- Malonyltryptophan in Higher Plants. 12
