Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene.
AUTOR(ES)
Hovi, T
RESUMO
Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovirus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells). Only polioviruses caused cytopathic effect over a 10-day period. Likewise, only polioviruses were isolated, by use of L alpha cells, from 168 fecal specimens from children from developing countries. These results suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=263705Documentos Relacionados
- Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene.
- Hibernoma formation in transgenic mice and isolation of a brown adipocyte cell line expressing the uncoupling protein gene.
- Simulation of human autosomal dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene.
- Polycythemia in transgenic mice expressing the human erythropoietin gene.
- Epstein-Barr virus (EBV) infection of murine L cells expressing recombinant human EBV/C3d receptor.