Selective probing of mRNA expression levels within a living cell
AUTOR(ES)
Nawarathna, D.
FONTE
American Institute of Physics
RESUMO
We report on a selective and nondestructive measurement of mRNA (messenger ribonucleic acid) expression levels within a living cell. We first modify an atomic force microscope tip to create a tapered nanoscale coaxial cable. Application of an ac (alternating potential) between the inner and outer electrodes of this cable creates a dielectrophoretic force attracting mRNA molecules toward the tip-end which is pretreated with gene specific primers. We selectively extracted and analyzed both high (∼2500) and extremely low (11¯0) copy number mRNA from a living cell mRNA in less than 10 s.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2749444Documentos Relacionados
- Sequences within the 5' untranslated region regulate the levels of a kinetoplast DNA topoisomerase mRNA during the cell cycle.
- mRNA analysis of single living cells
- Comparing protein abundance and mRNA expression levels on a genomic scale
- Selective inhibition of cell-free translation by oligonucleotides targeted to a mRNA hairpin structure.
- Autoregulatory control of translatable phytochrome mRNA levels