Semi-Micro, Dye-Binding Assay for Rabbit Interferon

AUTOR(ES)

Armstrong, John A.

RESUMO

The semi-micro method for interferon assay was based on quantitation of inhibition of cytopathic effects in 6-mm wells. Cultures were exposed to interferon dilutions, challenged with vesicular stomatitis virus, and stained with methylrosanaline chloride. The bound dye was then eluted and measured colorimetrically. The method was more sensitive than the plaque-inhibition method and was extremely economical. Culture age was the most important factor affecting reproductibility.

Documentos Relacionados

• Semi-Micro XC Cell Assay Technique for Murine Leukemia Virus
• Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods
• The Dye-binding Capacity of Human Plasma Determined Fluorimetrically and Its Relation to the Determination of Plasma Albumin
• Mode of Pisatin Induction: Increased Template Activity and Dye-binding Capacity of Chromatin Isolated from Polypeptide-treated Pea Pods 1
• SEMI-MICRO ESTIMATION OF REDUCING SUGARS